Induction of cellulase production by Sr2+ in Trichoderma reesei via calcium signaling transduction

Abstract Trichoderma reesei RUT-C30 is a well-known high-yielding cellulase-producing fungal strain that converts lignocellulose into cellulosic sugar for resource regeneration. Calcium ubiquitous secondary messenger regulates growth and cellulase production in T. . We serendipitously found adding Sr 2+ to the medium significantly increased activity upregulated expression of cellulase-related genes. Further studies showed supplementation cytosolic calcium concentration activated calcium-responsive signal transduction pathway Ca –calcineurin-responsive zinc finger transcription factor 1 (CRZ1). Using plasma membrane channel blocker, LaCl 3 , we demonstrated induces via signaling pathway. Supplementation with corresponding concentrations also inhibited colony growth. led an increase intracellular reactive oxygen species (ROS) transcriptional levels superoxide dismutase (s od1 ) catalase ( cat1 ). further ROS content was detrimental production, which alleviated by scavenger N -acetyl cysteine (NAC). This study first time stimulates upregulates genes leads ROS, can be NAC. Our results provide insights mechanistic synthesis discovery novel inducers cellulase. Graphical